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中国精品科技期刊2020
曲敏,李思桐,朱颖,等. Na+和大豆油对SPI乳液凝胶稳定性的影响及在素午餐肉中的应用J. 食品工业科技,2026,47(14):1−12. doi: 10.13386/j.issn1002-0306.2025110055.
引用本文: 曲敏,李思桐,朱颖,等. Na+和大豆油对SPI乳液凝胶稳定性的影响及在素午餐肉中的应用J. 食品工业科技,2026,47(14):1−12. doi: 10.13386/j.issn1002-0306.2025110055.
QU Min, LI Sitong, ZHU Ying, et al. Effects of Na+ and Soybean Oil on the Stability of SPI Emulsion Gels and Their Application in Vegetarian Luncheon MeatJ. Science and Technology of Food Industry, 2026, 47(14): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025110055.
Citation: QU Min, LI Sitong, ZHU Ying, et al. Effects of Na+ and Soybean Oil on the Stability of SPI Emulsion Gels and Their Application in Vegetarian Luncheon MeatJ. Science and Technology of Food Industry, 2026, 47(14): 1−12. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2025110055.

Na+和大豆油对SPI乳液凝胶稳定性的影响及在素午餐肉中的应用

Effects of Na+ and Soybean Oil on the Stability of SPI Emulsion Gels and Their Application in Vegetarian Luncheon Meat

  • 摘要: 蛋白质乳液凝胶的稳定性受蛋白质浓度、油体积分数、离子强度等多种因素的影响,限制了乳液凝胶的广泛应用。本文利用大豆分离蛋白(Soy protein isolate,SPI)和大豆油(Soybean oil,SO)制备乳液凝胶,研究SO和NaCl添加量对乳液凝胶稳定性的影响,考察质构特性、凝胶强度、持水持油性、水分分布、流变学特性、微观结构、蛋白质二级结构及分子间作用力等的变化,并将乳液凝胶应用于大豆蛋白基素午餐肉。结果表明:适量Na+和大豆油添加量显著增强了富含大豆油的SPI乳液凝胶的稳定性,特别是添加30% SO、0.434 mol/L Na+的凝胶,其表现出最佳的持水性、持油性(92.92%、96.47%)、弹性(0.90)、内聚性(0.90)(P<0.05),蛋白质二级结构从α-螺旋向β-折叠转变。将SPI、SO、Na+进行预处理制备的乳液凝胶应用于大豆蛋白基素午餐肉发现,预处理后的素午餐肉表面光滑,持水、持油性和弹性均优于对照。该研究为提高较高浓度的蛋白质-油乳液凝胶及乳状液滴聚集型凝胶的稳定性和凝胶强度,并应用于大豆蛋白基素肉产品开发中提供了理论参考。

     

    Abstract: The stability of protein emulsion gels is influenced by multiple factors, such as protein concentration, oil volume fraction, and ionic strength, limiting the widespread application of emulsion gels.In this study, emulsion gels were prepared using soy protein isolate (SPI) and soybean oil (SO) to investigate the effects of SO and NaCl addition on the stability of emulsion gels. Changes in textural properties, gel strength, water holding capacity, oil holding capacity, moisture distribution, rheological properties, microstructure, protein secondary structure, and intermolecular forces were examined, and the emulsion gels were applied to soy protein-based vegetarian luncheon meat. The results showed that appropriate amounts of Na+ and soybean oil significantly enhanced the stability of SPI emulsion gels enriched with soybean oil. In particular, with the addition of 30% SO and 0.434 mol/L Na+, the gels exhibited optimal water holding capacity, oil holding capacity (92.92%, 96.47%), springiness (0.90), and cohesiveness (0.90) (P<0.05). The protein secondary structure shifted from α-helix to β-sheet. SPI, SO, and Na+ were pretreated to prepare emulsion gels, which were subsequently incorporated into soy protein-based vegetarian luncheon meat. The pretreated vegetarian luncheon meat exhibited a smooth surface, and its water holding capacity, oil holding capacity and springiness to those of the control. This study provides theoretical insights for enhancing the stability and gel strength of high-concentration protein-oil emulsion gels and emulsion droplet aggregation-type gels, facilitating their application in soy protein-based vegetarian meat product development.

     

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