Abstract: Ampelopsis grossedentata is enriched with multiple biologically functional components, including flavonoids, phenolic acids, polysaccharides, amino acids , and other bioactive components. Dihydromyricetin is one of the key bioactive substances in Ampelopsis grossedentata. Ampelopsis grossedentata possesses various efficacies such as anti-inflammatory, antibacterial, antioxidant, anti-tumor, liver protection, regulation of blood glucose and blood lipids. It also exhibits promising prospects for application in the food field. Furthermore, an in-depth analysis of its application potential in processing fields such as functional food development, food preservation, and packaging is provided. By reviewing the mechanisms of action of the active compounds in Ampelopsis grossedentata and their research progress in food applications, this review clarifies their positive effects in regulating immune function, alleviating oxidative stress, inhibiting tumor cell proliferation, protecting liver function, and modulating glucose and lipid metabolism. This provides a scientific basis for the development of new healthy foods in protecting human health and preventing chronic diseases, while also laying a theoretical foundation for the high-value and industrial utilization of Ampelopsis grossedentata resources.