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中国精品科技期刊2020

负载姜黄素的玉木耳多糖乳液凝胶的制备及其体外消化性能研究

Preparation and In Vitro Digestibility of Auricularia cornea Polysaccharide Emulsion Gels Encapsulated with Curcumin

  • 摘要: 为了提升姜黄素在不同食品加工环境下的稳定性及其生物可及性,本研究以亚临界水萃取得到的玉木耳多糖(ACP)作为单一凝胶基质,以云南深纹核桃油为油相,通过单一的高速剪切制备了包埋姜黄素的玉木耳多糖基乳液凝胶。以姜黄素的包埋率、保留率为指标,评价了不同ACP用量下乳液凝胶对姜黄素的包埋保护性能,以液滴尺寸、ζ-电位、油滴形态和生物可及性为指标,测定了不同ACP用量下乳液凝胶对姜黄素消化性的影响。结果表明,天然提取未经改性的单一ACP构建的乳液凝胶对姜黄素具有很高的包埋作用,包埋率随多糖用量的增加而增大,当ACP的用量为1.0%(w/v)时包埋率为99.62%。ACP乳液凝胶对姜黄素的包埋作用显著提升了姜黄素在热处理、紫外线照射、碱性环境下的稳定性,其最高保留率分别为90.49%、94.88%和85.39%。在体外模拟消化过程中,包埋了姜黄素的ACP乳液凝胶在口腔、胃部基本保持稳定,在小肠部被消化形成含姜黄素的胶束,提高了姜黄素的生物可及性,最高达29.86%。研究表明,玉木耳多糖乳液凝胶不仅能有效包埋和保护姜黄素,还能提高其生物可及性。

     

    Abstract: To improve the stability and bioaccessibility of curcumin under diverse food processing environments, this study prepared emulsion gels for encapsulation of curcumin via one-step high speed shearing, using polysaccharides extracted from Auricularia cornea (ACP) and walnut oil. The encapsulation efficiency and retention rate of curcumin were measured to evaluate the protective effects of the ACP emulsion gels. The changes of oil droplets and surface zeta potential of the gels were analyzed during in vitro simulated digestion to assess their digestibility. Furthermore, the bioaccessibility of curcumin was determined. The results showed that emulsion gels formulated solely by ACP showed high encapsulation efficiency for curcumin. The encapsulation rate increased with increasing ACP concentrations, reaching an optimal value of 99.62% at an ACP concentration of 1.0% (w/v). The prepared curcumin-encapsulated ACP-based emulsion gels exhibited significant protective effects on curcumin under heating, ultraviolet irradiation, and pH conditions ranging from 1 to 12, with the highest retention rate of 90.49%、94.88% and 85.39%. During in vitro simulated digestion, these gels exhibited resistance to oral and gastric digestion, while curcumin-containing micelles were formed during the small intestinal phase. These micelles facilitated subsequent digestion and absorption of curcumin, thereby enhancing its bioaccessibility up to 29.36%. In summary, emulsion gels constructed solely with ACP are capable of protecting curcumin and improving its bioaccessibility through effective encapsulation.

     

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